Beating Embryonic Zebrafish Heart Imaged by Paired Alternating Acquisitions (PAAQ) Microscopy

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This dataset contains images of the beating heart of live embryonic zebrafish acquired using Paired Alternating AcQuisitions (PAAQ) microscopy. The dataset was collected to illustrate a multichannel video reconstruction method, which virtually increases the framerate of a repeating process (e.g., cardiac heartbeat) as compared to what would be achievable through direct imaging with a single-channel fluorescence setup and a low-framerate camera. The imaged embryos were obtained by crossing transgenic Tg(fli1a:GFP)y1Tg and Tg(myl7:mRFP)ko08Tg zebrafish and selecting homozygous embryos. The embryos were were imaged at 4dpf on an implementation of the OpenSPIM microscope equiped with and the illumination controller available as part of the CBI-MMTools microscopy controltools package for micromanager ( to generate alternating illumination patterns in even and odd frames. The odd frames correspond to illumination by a brightfield with intensity that increases as a ramp over the duration of the frame exposure (reference modality) and even frames are either the result of laser illumination (fluorescence imaging) or a short brightfield illumination burst.



 If you use this dataset, please cite the following publication :

Marelli, F., Ernst, A., Mercader, N., Liebling, M. (2023). PAAQ: Paired Alternating AcQuisitions for Virtual High Frame Rate Multichannel Cardiac Fluorescence Microscopy. Biological Imaging, 3, E20.